AQUATIC EXPERIMENTAL MICROBIOLOGY PLATFORM MICROBEX
MICROBEX is structured into 3 technical facilities that cover all needs of aquatic environmental microbiology: culture, observation, enumeration, measurement of functioning and dynamics of aquatic microorganisms.
-The Culture and Experimentation tray gathers equipments necessary for isolation, cultivation or breeding, and for the study of microorganisms ecophysiological processes from environmental samples or collections, to characterize their functioning in ecosystems.
-The Microscopy-Imaging tray gathers equipments tools of preparation and observation of samples of microorganisms of 0.02µm (e.g. virus) to 6.4 cm (e.g. fish larvae). It has a space dedicated to the preparation and storage of samples fixed for microscopic or macro observations, as well as spaces (light and dark) for optimal analysis of samples. Observed microorganisms have the ability to be sorted, counted, measured, identified and images scanned with the possibility of 3D re-creation or mosaic.
-The Flow Cytometry Flux Tray allows to count sort and physiologically characterize individual cells (30 nm to 20 µm) at high frequency (up to 50,000 cell/s), depending on their fluorescent-structure size (natural and/or induced) in response to a light stimulation (405-488-633 nm laser).
- Culture and experimentation:
Contact Claire Carré, the Culture and Experimentation tray manager, to assess needs and feasibility, and plan reservations for each space. It is possible to use the tray on a one-off basis or in a short to medium term (maximum 6 months). In the latter case, plan to organize yourself as soon as the projects are written to know the tray availability. A reception will then be made on the tray with a description of the good practices associated to it (plan for a DSR at least 15 days in advance). In addition, support in the implementation of manipulations and experiments can be carried out depending on the availability of the referee.
Contact Cécile Roques, the Microscopy-Imagery tray manager. First, a project feasibility study (technical and availability) will be carried out with the project holder. Methodological tests can be carried out before a Microscopy-Imaging (UM) tray estimate can be drawn up. Registration on paper plans must be done in consultation with the manager, at least 15 days before the start of the tray. Depending on the type of project and the availability of the reference, a service delivery job may be possible.
- Flow Cytometry:
Contact Cécile Roques, the flow cytometry tray manager to assess the needs, feasibility and type of machine to be used. After quotation is issued, a purchase order must be provided. The reservation (1 to 5j max) of the schedule must be made at least 15d in advance.